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KMID : 0043320050280121392
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Archives of Pharmacal Research
2005 Volume.28 No. 12 p.1392 ~ p.1399
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Protective Effects of -Tocopherol and Ischemic Preconditioning on Hepatic Reperfusion Injury
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Lee Woo-Yang
Lee Sun-Mee
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Abstract
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This study evaluated the effect of -tocopherol (-TC), ischemic preconditioning (IPC) or a combination on the extent of mitochondrial injury caused by hepatic ischemia/reperfusion (I/R). Rats were pretreated with -TC (20 mg/kg per day, i.p.) for 3 days before sustained ischemia. A rat liver was preconditioned with 10 min of ischemia and 10 min of reperfusion, and was then subjected to 90 min of ischemia followed by 5 h or 24 h of reperfusion. I/R increased the aminotransferase activity and mitochondrial lipid peroxidation, whereas it decreased the mitochondrial glutamate dehydrogenase activity. -TC and IPC individually attenuated these changes. -TC combined with IPC (-TC+IPC) did not further attenuate the changes. The mitochondrial glutathione content decreased after 5 h reperfusion. This decrease was attenuated by -TC, IPC, and -TC+IPC. The significant production of peroxides observed after 10 min reperfusion subsequent to sustained ischemia was attenuated by -TC, IPC, and -TC+IPC. The mitochondria isolated after I/R were rapidly swollen. However, this swelling rate was reduced by TC, IPC, and -TC+IPC. These results suggest that either -TC or IPC reduces the level of mitochondrial damage associated with oxidative stress caused by hepatic I/R, but - TC combined with IPC offers no significant additional protection.
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KEYWORD
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Ischemic preconditioning, Hepatic ischemia/reperfusion, Oxidative stress, Mitochondrial permeability transition
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